【摘要】 采用Hypersil CN(150 mm×4.6 mm,5μm)色谱柱,以甲醇-四氢呋喃-水(甲醇:四氢呋喃∶水=10∶10∶80,磷酸调pH=2.7)作为流动相,测定复方卡托普利片中卡托普利和氢氯噻嗪两种成分的含量.结果表明,卡托普利在50~250μg/ml范围内、氢氯噻嗪在26~130μg/ml范围内线性较好.卡托普利的平均回收率为100.7%,RSD=0.5%(n=5),氢氯噻嗪的平均回收率为100.5%,RSD=0.3%(n=5).该方法快速简捷、可靠、准确度高、重现性好,可同时测定复方制剂中两种成分.更多还原

【Abstract】 This research establishes an HPLC method for the determination of Captopril and Hydrochlorothiazide in compound Captopril Tablets.Determination is carried out on a Hypersil CN(150 mm × 4.6 mm 5 μm) Column with methanol-water as mobile phase and flow rate is 1.0 ml/min.Detection wavelength is at 220nm.Two good linearity are demonstrated by equation C=1.338×10-4A-2.563(n=5,r=0.9999)of Captopril and C=1.804×10-5A-1.997(n=5,r=0.9999)of Hydrochlorothiazide.The average recoveries of Captopril and Hydrochlorothiazide are 100.7% RSD=0.5%(n=5)nd 100.5% RSD=0.3%(n=5)seperately.This method is simple,reliable and accurate for simultaneous determination of Captopril and hydrochlorothiazide.更多还原