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降解豆粕菌株的筛选及发酵工艺优化
Strain screening for degradation of soybean meal and optimization of its fermentation technics
【摘要】 利用产蛋白酶能力较强的菌株将脱脂豆粕原料中的大豆蛋白降解为大豆多肽。以蛋白酶活力及水解度为指标筛选出降解豆粕能力较强菌株W-13,并以水解度为指标对菌株发酵降解豆粕的条件进行了优化,得到最适发酵条件为:11%豆粕、2%玉米粉、3%接种量、发酵时间48h,在最适培养基上蛋白水解度为65%。
【Abstract】 Defatted soybean meal is used as substrate and degraded to soybean polypeptides by the strain which could yield more proteinase.Taking proteinase activity and degree of hydrolysis as the key targets during strain screening,strain W-13 is screened out and used for defatted soybean meal degradation.The optimization conditions for W-13 fermentation are 11% of defatted soybean meal concentration,2% of corn flour concentration,3% of inoculate quantity and fermenting for 48 hours.The degree of hydrolysis reaches 65% on this culture medium.
【关键词】 豆粕;
发酵;
菌株筛选;
水解度(DH);
【Key words】 soybean meal; fermentation; strain screening; degree of hydrolysis(DH);
【Key words】 soybean meal; fermentation; strain screening; degree of hydrolysis(DH);
- 【文献出处】 粮食与食品工业 ,Cereal & Food Industry , 编辑部邮箱 ,2009年03期
- 【分类号】TS201.2
- 【下载频次】171