【摘要】 目的:观察艾灸加强药液穴位皮肤吸收法对博莱霉素所致肺纤维化大鼠肺组织形态学及生存状况的影响,探索其阻抑肺纤维化的治疗效应,为该法介入肺纤维化的治疗提供实验依据,并为进一步深入探索其作用机制打下实验基础。方法:选用SD大鼠以其气管内注入博莱霉素A5(BLMA5)制作肺纤维化模型,造模成功48只,随机分为4组:模型组?激素治疗组?灸药结合治疗组?单纯艾灸治疗组,每组12只;另设空白对照组12只。造模7天后开始治疗:灸药结合组采用在其"膏肓俞"和"肺俞"穴滴加"化纤方"药液再加以艾灸的方法,每次滴加化纤方药液5滴,艾灸至其穴位皮肤上的药液干掉为止(约10m in);单纯艾灸组只灸其"膏肓俞"和"肺俞"穴;激素治疗组每只腹腔注射5mg/kg泼尼松。以上均每日1次,连续治疗1个月。采用以下方法:(1)肉眼观察大鼠的食欲、神态、反应能力、皮毛、肌肉及呼吸情况并每10天称其体重,观察其肺组织的形状、色泽、表面状态、病灶特征,测其肺系数;(2)光镜观察其肺组织病理学改变。结果:(1)各组体重及生存数量存在较大差异,其中灸药结合治疗组最佳;(2)空白组肺组织基本属正常形态,灸药结合治疗组大鼠肺的基本情况较泼尼松组和单纯艾灸组优,模型组肺组织损害程度明显严重;(3)灸药结合治疗组、单纯艾灸组和泼尼松组肺水含量较模型组减少,其中灸药结合治疗组情况较优;(4)各组肺泡炎及肺纤维化程度分级比较结果提示:①造模比较成功;②灸药结合治疗组肺纤维化程度明显减轻。结论:灸药结合治疗法在一定程度上更能有效地阻抑大鼠肺纤维化的形成和发展,且与激素治疗相比较具有无毒副作用之优势。更多还原

【Abstract】 Objective:To investigate the effect of moxibustion reinforcing acupoint cutis to absorb liquid medicine on the pulmonary fibrosis of rats induced by BLMA5,to observe the influence on rats′lung tectology and status of life,to explore the therapeutic effect on pulmonary fibrosis,to provide experimental basis for treatment of huaxianfang and make a foundation of experimental basic for the further exploitation of mechanism of action.Methods:Animal model were successfully obtained by intratracheal injection with BLMA5 in SD rats.7 days later,rats were randomly divided into 4 groups:model group,Prednisone therapy group,Herb combined with moxibustion group,and Moxibustion group.In addition,12 healthy rats were set up as blank group.Each group will be received corresponding treatment respectively,once daily,30 consecutive days.Each group′s treatment is as followed:model group,no treatment;prednisone group,intraperitoneal injection with prednisone 5mg/kg;herb combined with moxibustion group,drop wised by HuaXianFang liquid followed by moxibustion at"GaoHuangShu"and"FeiShu"acupoint;moxibustion group,moxibustion at"GaoHuangShu"and"FeiShu"acupoint.(1)Researchers observe the rats′appetite,expression,reactive potency,clothing hair,muscle and breathby the unaided eye;keep a record of avoirdupois everyday;obseve the lung′s shape,the color of the surface coating of the mice,apparent condition,character of focus;test pulmonary coefficient.(2)Observing the pulmonary patholical changes by light microscope.Results:(1)There is a little large differences on the avoirdupois and the last number of live among Each group,meanwhile,herb combined with moxibustion group is the best.(2)The better lung tissue of rats are basicly normal in blank group,the fundamental state of lung in herb combined with moxibustion group compared with the Prednisone therapy group and moxibustion therapy group,the worst changes of lung tissue pathology is the model group.(3)The contained lung liquid of Prednisone therapy group,herb combined with moxibustion group,and moxibustion group is less than the model group,and,the herb combined with moxibustion group is the best.(4)The compared result of alveolar catarrh and pulmonary fibrosis shows that:①Making models was successful.②The remarkable alleviation of pulmonary fibrosis degree in herb combined with moxibustion group.Conclusion:The herb combined with moxibustion treatment is more effective in depression the come into being pulmonary fibrosis and it′s development in rats,and,compared with the hormone treatment group is less side-effect.更多还原