【摘要】 目的:观察玉米苞叶煎剂对食饵性高血脂大鼠主动脉内皮细胞凋亡的干预作用及血清NO、ET水平的影响。方法:选用雄性SD大鼠,随机分为正常对照、病理模型、阳性对照及玉米苞叶煎剂组。复制食饵性AS早期模型,同时给予玉米苞叶煎剂预防性给药,以吉非罗齐为阳性药对照。实验9周,检测血清脂质,NO和ET,流式细胞术定量检测主动脉内皮细胞凋亡率及增殖指数。结果:玉米苞叶煎剂组TC、TG、VLDL较模型组显著降低(P<0.05);内皮细胞凋亡率较模型组明显下降(P<0.05),增殖指数轻度上升(P>0.05);血清ET下降(P>0.05),NO上升(P>0.05)。结论:玉米苞叶煎剂可调节高脂大鼠血清NO和ET水平,降低内皮凋亡率,促进内皮修复。更多还原

【Abstract】 Objective:To study the effect of corn bract on the level of NO,ET and apoptosis of endothelial cells.Methods:Male Sprague-Dawley rats were randomly divided into control and pathological model,the positive control group and the decoction of corn bract.Early Atheros-clerotic models of hyperlipemia were developed by feeding the SD rats with high-cholesterol-fat diet and the decoction of corn bract were administrated to the rats at the same time.The blood-fat,NO and ET were measured after nine weeks,the rate of apoptosis and proliferation index(PI) of VEC were detected by the way of flow cytometry.Results:Corn bract could reduce the levels of serum TC,TG,VLDL as compared with the model group.The rate of apoptosis of VEC in corn bract group were lower than model group(P<0.05),but PI were higher(P>0.05).Corn bract could decreas the level of ET(P>0.05)and increase the level of NO(P>0.05).Conclusion:Corn bract can regulate the level of NO and ET,reduce endothelial apoptosis rate and promote endothelial repair.更多还原