【摘要】 研究衰老大鼠的学习行为与海马内突触相关蛋白的表达.运用Y-迷宫方法对幼年对照组(断乳期)和老年期(22月龄)雄性Sprague-Dawley大鼠进行Y-迷宫空间学习记忆行为训练,之后利用免疫组化和Western blot方法检测2组大鼠海马内突触素(synaptophysin,Syn)、生长相关蛋白(GAP-43)的表达情况.研究结果:(1)2组相比,老年组的学习能力较强,幼年对照组记忆能力较强(P<0.05);(2)生长相关蛋白GAP-43在老年组海马内的表达显著高于幼年对照组;而突触素的表达低于幼年对照组(P<0.01).提示,GAP-43和突触素可能参与大鼠空间分辨学习记忆的过程,老年大鼠的中枢神经系统保持着潜在的神经元退化修复的功能.更多还原

【Abstract】 To investigate the learning activity of the aging rats and the role of synaptic associated proteins in Y-maze bright-dark discrimination learning Male Sprague-Dawley rats of aged(22-month) group and young(weaning period) control group were trained by Y-maze and then the expressions of growth-associated protein 43(GAP-43) and synaptophysin(Syn) in hippocampus of the two groups rats were examined by immunohistochemistry and Western blot.Firstly,the results showed that the learning ability of the aged group rats was stronger while the memory ability of the young control group rats was stronger.Secondly,the expression of GAP-43 in hippocampus of the aged group was greater than that of the young control group.However,from Western blot results,we found that lower expression of Syn was shown in the hippocampus of the aged group.These results suggest that GAP-43 and Syn may be involved in the process of Y-maze discriminative learning and memory and the potential function of repairing degenerative neurons in the aged brain might be remained in the rats.更多还原