【摘要】 目的:利用拉曼光谱在分子水平上研究宫颈重度上皮内瘤变组织与宫颈癌组织病变细胞与正常细胞的差别。方法:取手术切除或活检的宫颈重度上皮内瘤变及宫颈鳞癌各20例,并取其相应病变旁正常组织作为对照组。利用显微共焦拉曼光谱仪测定病变组织,以及其(和)相应病变旁相对正常组织的光谱。结果:病变组织和病变旁组织的显微共焦拉曼光谱差异有显著性,癌组织具有特征峰。结论:宫颈癌病变组织与癌旁相对正常组织的拉曼光谱均有明显差异,拉曼光谱仪可以用来判断宫颈癌病变与正常宫颈组织,有望成为宫颈癌早期诊断的新方法。更多还原

【Abstract】 Obiective:Use Raman Spectrum technology was performed on cervical invasive squamous cell carcinoma tissues and cervical intraepithelial neoplasiaⅢ,as well as their surrounding normal tissues.The spectroscopic studies together provide the difference between the chemical molecular structure of the cervical cancer and cervical intraepithelial neoplasia tissues.Methods:20 samples of cervical invasive squamous cell carcinoma tissues and cervical intraepithelial neoplasiaⅢtissues and normal tissue surrounding them were taken during surgery or direct biopsy.Use Raman spectrum technology was performed on cervical invasive squamous cell carcinoma tissues and cervical intraepithelial neoplasiaⅢtissues,as well as their surrounding normal tissues.Results:There is spectral band variability of the cervical lesion tissue and surrounding normal tissues and have the characteristic wave in cervical cancer tissues.Conclusion:The lesion tissue of Raman spectrum which have obvious difference,it is shown the difference in both molecular composition and structure.Therefore,using the spectroscope to diagnose the lesion tissue and normal cervical tissue,to provide the reference as the new method to diagnose the cervical cancer early stage.更多还原