【摘要】 先用蒸汽蒸馏法和乙醚萃取法将白丁香花中的挥发性组分从样品中分离,在所得萃取液中加无水硫酸钠脱水后,在水浴上蒸发除去乙醚,所得淡黄色的精油采用两种不同极性的色谱柱(DB-17及HP-5MS)进行气相色谱分离和用质谱法进行鉴别和测定。结果表明:两种色谱柱分离的产物中有46种主要组分基本相同。但在经DB-17柱分离的产物中有两种化合物和在经HP-5MS柱分离的产物中有一种化合物分别在另一柱的产物中未能找到。采用提取离子法和数据分析软件中提供的峰纯度检查法对上述问题作了进一步考查,结果表明:这3种化合物实际上都存在,只是由于色谱的重叠或交叉而未能予以鉴别。显然,用单一色谱柱可用于挥发油主成分的分离,但对复杂体系挥发油组分的更好分离和更准确的鉴别和测定,选用不同极性的色谱柱进行同时分离和校核更为有利,而采用上述两种方法作进一步的考查也十分必要。更多还原

【Abstract】 Volatile components in white clove flower were separated from the sample by steam distillation and ether extraction successively.After dehydration with anhydrous Na₂SO₄,the extract was evaporated on a water bath to drive off ether.The lightly yellow colored floral essence obtained was separated by GC separately on 2 chromatographic columns with different polarity,i.e.,the DB-17 and HP-5MS columns,for its componental compounds which were identified and determined by MS.It was found that 46 main components were found in both of the products separated by DB-17 column and HP-5MS column.But 2 compounds which were separated by the DB-17 column,were not found in the products from HP-5MS column,while one compound separated by HP-5MS was not found in products from DB-17.In a further study by using the EIC method and the method of peak purity review of the data analysis soft ware,the existence of the 3 compounds was confirmed.Evidently,although a single chromatographic column is feasible to be used for the separation for the main volatile components of the floral essence,but for more complete separation and more accurate identification,use of 2 colunms with different polarity is more preferrable,and the help of the methods for further confirmation is also necessary.更多还原