【摘要】 基于在磷酸介质中,钼（Ⅵ）催化过氧化氢氧化碘化钾产生游离碘,并进一步与吖啶橙反应导致其荧光猝灭,据此提出荧光光度法测定痕量钼（Ⅵ）的一种灵敏的方法。采用固定时间法测得在446 nm(λ_ex)及505 nm(λ_em)波长处反应体系的△F（即F₀与F值之差）与钼（Ⅵ）的质量浓度在0.16～2.4μg·L^-1范围呈线性关系。方法的检出限（3s/k）为0.10μg·L^-1。该方法应用于绿豆、茶叶和矿泉水中钼的测定,测得方法的回收率在93%～99%之间,测定值的相对标准偏差（n=5）均小于5%。更多还原

【Abstract】 Based on the catalytic action of Mo（Ⅵ） on the oxidation of KI by H₂O₂ in dilute H₃PO₄ medium to give free iodine（in form of I₃^-） which in turn reacted with acridine orange leading to quenching of fluorescence of acridine orange,a fluorophotometric method for determination of Mo（Ⅵ） was proposed.Linear relationship was found between values ofΔF（F₀-F） determined at wavelengths of 446 nm(λ_ex) and 505 nm(λ_em) and concentration of Mo（Ⅵ） in the range of 0.16-2.4μg·L^-1.Limit of detection（3s/k） of the method found was 0.10μg·L^-1. The proposed method was used in the determination of Mo（Ⅵ） in samples of green bean,tea and mineral water, giving values of recovery ranged from 93%-99%,and of RSD’s（n=5） less than 5%.更多还原