【摘要】 目的:利用我们前期构建的溶瘤腺病毒SG600技术平台,研究其携带野生型p53基因对裸鼠胃癌移植瘤模型抗肿瘤活性。方法:克隆p53基因全长cDNA,插入到溶瘤腺病毒SG600基因组中,同时建立裸鼠胃癌移植瘤模型,体内观察SG600-p53对胃癌移植瘤模型的抗肿瘤疗效。结果:成功构建溶瘤腺病毒SG600-p53,裸鼠胃癌移植瘤的p53基因获增强表达。在胃癌移植瘤裸鼠模型中,经4周治疗,重组SG600-p53抑瘤率达75.59%,优于Ad-p53(45.00%)和SG600(56.95%)。结论:SG600-p53作为一种有效的抗肿瘤新型制剂,对胃癌移植瘤有很好的特异性抗肿瘤活性,可能具有良好的临床应用前景。更多还原

【Abstract】 Objective:By using the oncolytic adenovirus SG600 constructed previously,the antitumor activity of the p53 gene-enhanced oncolytic adenovirus SG600-p53 was examined in gastric cancer xenografts in nude mice.Methods:The full-length p53 cDNA was cloned and inserted into the genome of oncolytic adenovirus SG600.The antitumor activity of SG600-p53 was observed in a nude mice model of human gastric cancer.Results:SG600-p53 could selectively kill gastric cancer cells,and inhibit the growth of gastric cancer xenografts in nude mice,with the tumor inhibition rate of 75.59%.Conclusion:SG600-p53 is a novel effective antitumor agent with better antitumor activity in gastric cancer xenografts in nude mice,and may have a splendid future in clinic.更多还原