【摘要】 目的观察罗格列酮对肝星状细胞增殖与Ⅰ型胶原合成的影响。方法采用HSC-T6肝星状细胞系,将培养的肝星状细胞(HSC)分为对照组、不同浓度的罗格列酮组(5、10、20μmol/L)。采用MTT法观察罗格列酮对HSC增殖的影响,采用ELISA法检测细胞培养液上清中Ⅰ型胶原的含量。结果与对照组相比,在10、20μmol/L罗格列酮组,罗格列酮可以抑制HSC的增殖(P<0.05),同时细胞培养液上清中I型胶原含量分别为135.72±6.00ng/ml、128.65±5.34ng/ml,均低于对照组(149.35±6.65ng/ml),差异有统计学意义(P<0.05)。结论罗格列酮可以抑制HSC的增殖,抑制肝星状细胞I型胶原的合成。更多还原

【Abstract】 Objective To investigate the effects of rosiglitazone on the proliferation and collagen I synthesis of cultured hepatic stellate cells(HSC)in vitro.Methods HSC line,HSC-T6 was incubated with different concentraion of rosiglitazone(5,10,20 μmol/L).Cell proliferaeion was examined by MTT colormetric assay.Cell culture medium was used to detect collagen I level with ELISA method.Results Compared to control group,10,20 μmol/L rosiglitazone groups can inhibit HSC proliferation(P<0.05).The collagen I level of 10,20 μmol/L rosiglitazone groups were 135.72±6.00 ng/ml,128.65±5.34 ng/ml respectively,which were significantly lower than control group(149.35±6.65 ng/ml,P<0.05).Conclusion Rosiglitazone can inhibit HSC proliferation,decrease the collagen I synthesis.更多还原