【摘要】 目的:观察急性血栓性肺栓塞(acute pulmonary thromboembolism,PTE)兔血清中肝细胞生长因子(hepatocyte growth factor,HGF)的变化特征,并探讨其对PTE的早期诊断价值。方法:新西兰纯种白兔16只,随机均分为对照组和模型组;模型组采用自体血栓回输法建立PTE模型,对照组以等量0.9%氯化钠溶液代替。分别在0、1、3、6、12、24、48、72h采集血样,ELISA法检测HGF及IL-1β含量。结果:模型组血HGF水平于1h后开始升高[(0.853±0.317)μg/L],持续升高到3h略降低后又继续升高,12h升高最明显[(1.742±0.487)μg/L],在48h仍保持高水平,72h开始回落;对照组各时间点血HGF水平均无明显变化,一直保持在较低水平;模型组在1、3、6、12、24、48、72h血清HGF水平均显著高于对照组(P<0.05～0.01)。模型组血IL-1β于0h后开始升高,在3h达到峰值(0.181μg/L),6h降至较低水平(0.09μg/L),直至72h无明显变化;对照组始终在<0.089μg/L水平。结论:HGF有望作为一种新的生物学标记物用于PTE早期诊断。更多还原

【Abstract】 Objective:To study the feature of hepatocyte growth factor (HGF) in the plasma of rabbit with acute pulmonary thromboembolism(PTE),and to explore the early diagnostic value to PTE. Method:Total of 16 rabbits were randomly divided into blank and PTE groups with 8 in each group,the PTE model was estabished by intravenous injection autologous blood clots,the sham-opreated groups was instead with NS. The level of HGF and IL-1β (0,1,3,6,12,24,48,72 h) was detected by ELISA. Result:The plasma HGF level between PTE group and blank group was signaficant(P<0.05),and began to elevated at 1 h after PTE,HGF was markedly increased at 6 h,12 h,24 h and 48 h in PTE groups,signaficantly elevated at 12 h([1.742±0.487]μg/L,P<0.01),then deceased at 72 h. The blank groups was below 0.5 μg/L.IL-1βreached the maximum level at 3 h (0.181 μg/L).Conclusion:The HGF may be a new biol-marker for the early diagnosis of PTE.更多还原