【摘要】 目的:探讨术前动脉化疗栓塞对膀胱癌组织Ki-67和增殖细胞核抗原(PCNA)表达的影响及其临床意义。方法:对30例膀胱癌患者化疗栓塞前后的肿瘤组织,应用免疫组织化学法测定Ki-67和PCNA的表达,并分析Ki-67和PCNA与膀胱癌病理分级和临床分期的关系。结果:对30例膀胱癌患者化疗栓塞前后的肿瘤组织,应用免疫组织化学法测定Ki-67和PCNA的表达,并分析Ki-67和PCNA与膀胱癌病理分级和临床分期的关系。结论:化疗栓塞前后Ki-67中高度阳性表达率为70%、26.67%,PCNA为73.33%、20%,差异均有非常显著性意义(P<0.01),经随访24.6个月,复发率为16.67%。Ki-67和PCNA阳性表达及下降幅度与膀胱癌的病理分级、临床分期和患者术后复发率关系密切,两者阳性表达及表达强度呈正相关(P<0.001)。结论:术前化疗栓塞能降低膀胱癌组织Ki-67和PCNA的表达,调节膀胱癌的分化程度,使肿瘤降级降期,减少术后转移,降低复发率,提高生存率。Ki-67和PCNA的表达可作为膀胱癌预后估计的指标。更多还原

【Abstract】 Objective:To investigate the influence and clinical significance of preoperative arterial chemoembolization on the expression of Ki-67 and proliferating cell nuclear antigen(PCNA) in bladder cancer tissue.Methods: Before and after chemoembolization of therapy,the expression of Ki-67 and PCNA count in in bladder cancer tissue of 30 patients were examined by immunohistochemical staining.The relation of its expression to the pathological grade and clinical stage was evaluated clinically.Results:Positive rate of Ki-67 expression in the tissue of bladder cancer were 70%and 26.67%respectively,PCNA were 73.33%and 20%.There were a very significant difference between the before and after therapy(P<0.01).Follow-up for mean 24.6 months recurrent rate in 30 cases was 16.67%.The positive expression and decline range of Ki-67 and PCNA correlated to the pathological grade, clinical stage and patient recurrent rates of bladder cancer.The positive expression and expression intensity of Ki- 67 correlated with PCNA(P<0.001).Conclusions:Preoperative arterial chemoembolization can reduce the expression of Ki-67 and PCNA,adjust malignancy of bladder cancer,could downgrade the tumors,and cut down the postoperative metastasis,improving survival rate and reducing recurrence rate.Both of the Ki-67 and PCNA labeling index are predictive factor for prognosis of bladder cancer.更多还原