【摘要】 目的:探讨表皮生长因子(Epidermal growth factor,EGF)和口腔鳞癌侵袭转移的关系。方法:采用发色底物反应法测量不同浓度EGF作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后u-PA和PAI-1的活性,同时用Boyden Chamber观察EGF诱导口腔鳞癌TSCCa和GNM细胞转移作用。结果:不同浓度的EGF(0ng/ml,10ng/ml,20ng/ml,40ng/ml,80ng/ml)作用于两种细胞系后,随着外源性EGF浓度的增加,GNM和TSCCa细胞中u-PA和PAI-1的活性均有增加,与对照组相比差异有显著性(p<0.05);而以TSCCa细胞中u-PA和PAI-1的活性增加较为明显,u-PA和PAI-1活性与外源性EGF有剂量依赖关系,用不同浓度10ng/ml,20ng/ml,40ng/ml,80ng/ml的EGF培养TSCCa和GNM细胞2h,GNM和TSCCa细胞穿过滤膜数均有增加,在相同的浓度内,TSCCa细胞较GNM细胞增加较为明显,两者相比差异有显著性(p<0.05)。结论:EGF可促进口腔鳞癌细胞侵袭转移。更多还原

【Abstract】 Objective:To detect the role of epidermal growth factor(EGF)on the invasiveness and metastasis of hu-man oral squamous cell carcinoma.Method:Using chromogenic substrate assay to determine the activity of u-PA and PAI-1 in oral squamous cell carcinoma cell lines TSCCa and metastasis carcinoma cell lines GNM treated by different levels of EGF.Boyder chamber were used to observe the function of metastasis in oral squamous cell carcinoma cells TSCCa and GNM.Result:The different levels of EGF(0 ng/ml,10 ng/ml,20 ng/ml,40 ng/ml,80 ng/ml)had increased the ac-tivity of u-PA and PAI-1in TSCCa and GNM cell lines,Moreover,the activity of u-PA and PAI-1had more dependent on EGF.The cells in lower chamber were increased after cultured for 2 hours by different levels of EGF,which were remarked higher in TSCCa cell lines than that of in GNM cell lines(P<0.05).Conclusion:EGF may be a factor associated with inva-sive and metastasis potential of oral squamous cell carcinoma.更多还原