【摘要】 目的探讨自身免疫性溶血性贫血(AIHA)患者外周血转化生长因子β1(TGF-β1)和白细胞介素10(IL-10)水平变化及临床意义。方法采用酶联免疫吸附测定(ELISA)方法对30例AIHA患者治疗前后和15例健康对照者血浆TGF-β1和IL-10的浓度进行检测。结果30例AIHA患者治疗前血浆TGF-β1水平与健康对照组比较明显降低(194.41±22.36)ng/Lvs(285.01±15.50)ng/L(P<0.01),治疗后血浆TGF-β1水平与治疗前比较显著升高(246.43±26.52)ng/L(P<0.01),但仍低于正常人(P<0.01)。AIHA患者治疗前血浆IL-10的水平与健康对照组比较明显升高(111.44±12.04)ng/Lvs(53.77±5.76)ng/L(P<0.01),治疗后血浆IL-10水平与治疗前比较显著降低(67.05±17.27)ng/L(P<0.01),但仍高于正常人(P<0.01)。AIHA患者治疗后完全缓解者和部分缓解者TGF-β1的水平与治疗无效者比较明显升高(264.93±9.69)ng/Lvs(207.50±16.53)ng/L,(256.96±8.35)ng/Lvs(207.50±16.53)ng/L(均P<0.01),但都仍明显低于健康对照者(P<0.01)。完全缓解者和部分缓解者IL-10水平与治疗无效者比较均明显降低(57.91±4.54)ng/Lvs(96.54±4.74)ng/L,(57.43±5.12)ng/Lvs(96.54±4.74)ng/L(均P<0.01),完全缓解者和部分缓解者IL-10水平与健康对照者比较差异无统计学意义(P>0.05),治疗无效者IL-10的水平与健康对照者比较升高(96.54±4.74)ng/Lvs(53.77±5.76)ng/L(P<0.01)。结论AIHA患者治疗前后血浆TGF-β1和IL-10水平的变化,提示TGF-β1和IL-10可能参与了该疾病的发生发展。更多还原

【Abstract】 Objective To explore the changes of transforming growth factor-β1(TGF-β1) and interleukin-10(IL-10) in patients with autoimmune hemolytic anemia(AIHA) with clinical significance.Methods Using ELISA method,the plasma levels of TGF-β1 and IL-10 were detected in 30 patients with AIHA before and after treatment and 15 healthy normal controls.Results The plasma level of TGF-β1 was higher after treatment than before in AIHA patients,(246.43±26.52) ng/L vs (194.41±22.36) ng/L(P<0.01),both of which were significantly lower than that of normal controls,respectively,(246.43±26.52) ng/L vs (285.01±15.50) ng/L (P<0.01) and (194.41±22.36) ng/L vs (285.01±15.50) ng/L(P<0.01).The plasma level of IL-10 was lower after treatment than before in AIHA patients,(67.05±17.27) ng/L vs (111.44±12.04) ng/L(P<0.01),both of which were significantly higher than that of normal controls,respectively,(67.05±17.27) ng/L vs (53.77±5.76) ng/L(P<0.01) and (111.44±12.04) ng/L vs (53.77±5.76) ng/L(P<0.01).The plasma levels of TGF-β1 were significantly higher in complete remission patients and partial remission than that in no-remission patients (264.93±9.69) ng/L vs (207.50±16.53) ng/L,(256.96±8.35) ng/L vs (207.50±16.53) ng/L(both P<0.01).The plasma levels of IL-10 were lower in complete remission patients and partial remission than that in no-remission patients (57.91±4.54) ng/L vs (96.54±4.74) ng/L,(57.43±5.12) ng/L vs (96.54±4.74) ng/L(both P<0.01).Conclusion TGF-β1 and IL-10 may be involved in pathophysiology of AIHA.更多还原