【摘要】 目的探讨自身免疫性溶血性贫血(AIHA)患者外周血CD4+CD25+调节性T细胞水平及临床意义。方法采用流式细胞仪对25例AIHA患者治疗前后和15例正常对照者外周血CD4+CD25+调节性T细胞占CD4+T细胞的比例进行了检测。结果25例AIHA患者治疗前CD4+CD25+调节性T细胞占CD4+T细胞的比例与正常对照组比较明显降低,(4.47±2.11)%vs(9.82±2.98)%(P<0.001),治疗后CD4+CD25+调节性T细胞的比例与治疗前比较显著升高,(7.60±2.50)%vs(4.47±2.11)%(P<0.001),但仍低于正常人,(7.60±2.50)%vs(9.82±2.98)%(P<0.05)。治疗后完全缓解患者CD4+CD25+调节性T细胞占CD4+T细胞的比例与治疗无效组比较明显升高,(9.55±2.36)%vs(5.68±2.67)%(P<0.05),无效组患者CD4+CD25+调节性T细胞占CD4+T细胞的比例与正常对照组比较仍然降低,(5.68±2.67)%vs(9.82±2.98)%(P<0.01)。结论AIHA患者外周血CD4+CD25+调节性T细胞的比例较正常对照明显降低,有效治疗后得到恢复,提示CD4+CD25+调节性T细胞可能参与了疾病的发生。更多还原

【Abstract】 Objective To explore the change of CD4~+CD25~+ regulatory T cells and its clinical significance in patients with autoimmune hemolytic anemia(AIHA).Methods The frequencies of CD4~+CD25~+ regulatory T cells were detected in 25 patients with AIHA before and after treatment and 15 normal controls by multi-colour flow cytometry.Results The proportion of CD4~+CD25~+ regulatory T cells in CD4+ T cells was higher after treatment than before in AIHA patients,(7.60±2.50)% vs(4.47±2.11)%(P<0.001),both of which were significantly lower than that of normal controls,respectively,(7.60±2.50)% vs(9.82±2.98)%(P<0.05),(4.47±2.11)% vs(9.82±2.98)%(P<0.001).The proportion of CD4~+CD25~+ regulatory T cells in CD4+ T cells was significantly lower in no-remission patients than that of normal controls,(5.68±2.67)% vs(9.82±2.98)%(P<0.05).Conclusion CD4~+CD25~+ regulatory T cells may be involved in pathophysiology of AIHA.更多还原