【摘要】 目的:体外观察塞来昔布对人鼻咽癌CNE-2细胞增殖和凋亡的影响。方法:采用MTT比色法观察其对鼻咽癌细胞生长的影响;透射电子显微镜检测细胞凋亡,应用流式细胞术观察DNA的含量和凋亡的变化情况,TUNEL染色法计数细胞凋亡指数。结果:体外塞来昔布抑制CNE-2细胞生长,呈浓度和时间依赖性。透射电镜观察到细胞皱缩、胞质丢失、核质浓缩以及凋亡小体形成等凋亡形态学改变。流式细胞术显示细胞凋亡率显著增高,在80、100μmol/L浓度下细胞凋亡率分别为(10.47±0.18)%和(20.17±0.55)%,与对照组(1.57±0.27)%比较差异均有统计学意义;塞来昔布使G0/G1期细胞比例升高,S和G2/M期比例下降,呈一定剂量效应关系。TUNEL染色法显示药物组凋亡率明显高于对照组(P<0.01)。结论:塞来昔布能抑制人鼻咽癌CNE-2细胞增殖,并诱导其凋亡;其机制可能与诱导凋亡和阻止细胞周期进展有关。更多还原

【Abstract】 Objective:To detect the effect of Celecoxib on the proliferation and apoptosis of human nasopharyngeal carcinoma cell line CNE-2.Method:The growth inhibition rate of CNE-2 by Celecoxib was evaluated with MTT method. Apoptosis related morphology changes were observed with transmission electron microscopy (TEM). The cell cycle and apoptosis were measured with flow cytometric method (FCM). Apoptotic index (AI) was counted by the TDT-mediated dUTP-biotin nick end-labeling (TUNEL) assay.Result:The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner. Apoptosis with nuclear chromatin condensation,cell shrinkage,periplast loss and the formation of apoptotic bodies was observed with TEM. Apoptotic rates of CNE-2 cells treated with 80 and 100 μmol/L celecoxib were (10.47±0.18)% and (20.17±0.55)% respectively,significantly higher than those of the control group (1.57±0.27)% with FCM.The percentage of G0/G1 phase cells increased,whereas the S and G2/M phases cells decreased in a dose-dependent manner after the treatment. TUNEL assay showed that the apoptosis ratio(AI)of CNE-2 treated with Celecoxib was higher than control group(P<0.01).Conclusion:Celecoxib can inhibit the growth of human nasopharyngeal carcinoma cell line CNE-2 and induce the cell apoptosis,which may be related to blocking the cell cycle progress of CNE-2 cells.更多还原