【摘要】 目的:探讨p53、p21蛋白在鼻腔NK/T细胞淋巴瘤(nasal NK/T-cell lymphoma,NKTL)中的表达,及其与细胞增殖和凋亡的关系。方法:取62例NKTL组织构建组织芯片,应用免疫组织化学方法检测p53、p21、Ki67蛋白的表达情况,TUNEL法原位凋亡检测,计算增殖指数(proliferation index,PI)和凋亡指数(apoptosis in-dex,AI)。结果:①p53、p21蛋白在NKTL中表达的阳性率分别为79.03%、58.06%;②p53在Ann ArborⅠ、Ⅱ、Ⅲ、Ⅳ期患者中表达的阳性率分别为69.57%、75.00%、86.67%、100.00%,p21在Ann ArborⅠ、Ⅱ、Ⅲ、Ⅳ期患者中表达的阳性率分别为47.83%、56.25%、60.00%、87.50%,随着肿瘤进展,二者表达阳性率逐渐升高,差异有统计学意义(P<0.05);③p53在大、中、小型瘤细胞患者中表达的阳性率分别为92.86%、78.95%、53.33%,p21在大、中、小型瘤细胞患者中表达的阳性率分别为67.86%、57.89%、40.00%,随着肿瘤细胞增大,二者表达阳性率逐渐升高,差异有统计学意义(P<0.05);④p53和p21二者表达之间统计学上具有明显的相关性(P<0.05);随着p53、p21蛋白表达强度的增加,肿瘤临床分期增高,肿瘤细胞增大,PI亦逐渐升高,它们均与PI呈正相关(P<0.05),而与AI无关联性(P>0.05)。结论:p53、p21蛋白表达与NKTL的发生、发展密切相关,联合检测Ki67表达,可作为评估该病患者肿瘤细胞增殖、侵袭性等生物学行为的良好指标。更多还原

【Abstract】 Objective:To investigate the significance of expressions of p53 and p21 in nasal NK/T-cell lymphoma(NKTL) and their relationship with cell proliferation and apoptosis.Method:Sixty-two cases of NKTL were examined for p53,p21 and Ki67 proteins by means of tissue microarray technique,TUNEL and immunohistochemistry.The proliferation index(PI) was determined by expression of Ki67 proteins.Result:The positive expression rates of p53 and p21 proteins in NKTL were 79.03% and 58.06% respectively.The positive expression rates of p53 in Ann Arbor stage Ⅰ,Ⅱ,Ⅲ and Ⅳ NKTL were 69.57%,75%,86.67% and 100% respectively,while those of p21 were 47.83%,56.25%,60% and 87.50%.With the progression of tumor,the positive expression rates of p53 and p21 proteins gradually increased.And there were significant differences between them(P<0.05).The positive expression rates of p53 in NKTL with large,medium and small size tumor cells were 92.86%,78.95% and 53.33% respectively,while those of p21 were 67.86%,57.89% and 40.00%.With the expanding of tumor cells,the positive expression rates of p53 and p21 proteins gradually increased.And there were significant differences between them(P<0.05).The expression of p53 was positively correlated with the expression of p21(P<0.05).The intensity of p53 and p21 expression,the Ann Arbor stage and the size of tumor cell all were positively correlated with PI(Spearman correlation analysis,P<0.05),while no correlation with AI(P>0.05).Conclusion:The expressions of p53,p21 and Ki67 proteins are closely related with the pathogenesis and progression of NKTL.Combined detection of p53,p21 and Ki67 is a good marker to judge the biological behavior of NKTL,such as the proliferation and the invasiveness of the tumor.更多还原