【摘要】 目的探讨应用靶向超声分子成像探查心肌缺血再灌注损伤炎症"印记"的可行性。方法采用"亲和素-生物素"桥接法构建携唾液酸化路易斯（Sialyl Lewis^X）靶向超声微泡(MB_sLex)和同型对照微泡（MBc）。10只心肌缺血再灌注小鼠随机先后注入MB_sLex和MBc（间隔30 min）,分别于注入5 min后行心肌对比超声检查,测量心肌缺血区和非缺血区的声强度（Ⅵ）。结果对比超声图像显示MB_sLex组缺血区心肌造影见显著增强,Ⅵ值高达23.52±1.08,而在MBc组缺血区心肌造影仅见轻度增强,Ⅵ值为9.81±0.41,两者之间差异有统计学意义（P<0.05）。但无论MB_sLex组还是MBc组,缺血区心肌Ⅵ值均明显高于非缺血区心肌Ⅵ值（P<0.05）。两组非缺血区心肌之间Ⅵ值未见明显差异。结论应用携sLex超声微泡行对比超声能够靶向探查心肌缺血再灌注损伤的炎症"印记"。更多还原

【Abstract】 Objective To explore the feasibility of inflammatory"imprint"in myocardial ischemical reperfusion injury detected by targeted myocardial contrast echocardiography（MCE）.Methods Targeted ultrasound microbubbles with sialyl Lewis^X （sialyl Lewis^X,sLex） and isotype control microbubbles were conjugated by"avidin-biotin"bridge.10 mice with myocardial ischemical reperfusion were injected with MB_sLex and MBc randomly（interval was 30 min）,then examined by myocardial contrast echocardiography（MCE） after 5 min,the video intensity（Ⅵ） was determined.Results Contrast echocardiography images showed ischemic region myocardium was enhanced obviously in MB_sLex group,Ⅵwas（23.52±1.08）,and ischemic region myocardium was enhanced in group MBc withⅥof（9.81±0.41）,there was significant difference between the two groups（P<0.05）.TheⅥof ischemic region myocardium was obviously higher than that in non-ischemic myocardium（P<0.05）.There was no significant difference inⅥbetween the two groups.Conclusion Molecular imaging of selectin with targeted contrast echocardiography is possible to detect myocardial ischemia-reperfusion injury effectively.更多还原