【摘要】 目的探讨Th17细胞与CD4+CD25+调节性T细胞在儿童过敏性紫癜(HSP)免疫发病机制中的作用。方法过敏性紫癜急性期患儿48例,采用流式细胞术检测外周血CD4+CD25+调节性T细胞(CD4+CD25+Treg)的比例,采用荧光定量PCR(real-time PCR)检测CD4+ T细胞IL-17A、IL-17F、转录因子ROR-γt、Foxp3及细胞因子IL-6、TGF-β等mRNA表达;同期40例同龄健康儿童作为对照。结果过敏性紫癜急性期患儿CD4+ T细胞高表达IL-17A及IL-17F(P<0.01)。Th17细胞转录因子ROR-γt及前炎症细胞因子IL-6 mRNA表达明显增高(P<0.01)。CD4+CD25+调节性T细胞比例明显低于正常对照组(P<0.01),其转录因子Foxp3表达亦明显降低(P<0.01)。结论Th17等促炎性T细胞高表达和CD4+CD25+调节性T细胞数量减少导致的免疫抑制效应不足,是导致过敏性紫癜免疫失衡的重要原因,IL-6高表达与此密切相关。更多还原

【Abstract】 Objective To investigate the roles of Th17 cells and CD4 +CD25 + Treg cell in immunological pathogenesis of Henoch-Schnlein purpura(HSP).Methods Forty-eight patients with HSP and 40 age-matched healthy subjects were recruited.Flow cytometric analysis(FCM) was performed to detect the percentage of CD4+CD25+Treg cells subpopulation.real-time PCR were used to analyze expression of IL-17A,IL-17F,ROR-γt,Foxp3,IL-6,TGF-β in CD4+ T cell.Results Compared with healthy control subjects,expressions of IL-17A and IL-17F were significantly upregulated during the acute phase of HSP(P < 0.01),expressions of the transcription factor ROR-γt and proinflammatory cytokine IL-6 in CD4+ T cells were significantly higher during the acute phase of HSP(P < 0.01),the proportions of CD4+ CD25+Treg were significantly lower in patients with HSP(P < 0.01).The mRNA expression of transcription factor Foxp3 showed similar tendency in patients with HSP(P < 0.01).Conclusions Aberrant activation of Th17 cell and the decrease of CD4+CD25+Treg cell might be involved in pathogenesis of HSP,which suggest that it might be associated with the increase expression of IL-6.更多还原