【摘要】 目的研究ACE基因插入/缺失(I/D)多态性与云南彝族原发性高血压的相关性.方法采用聚合酶链式反应(PCR)技术,检测云南省晋宁县194例彝族高血压患者和129例彝族正常人的ACE基因I/D多态性,计算DD、ID、II基因型频率和D、I等位基因频率,并对结果进行相关性分析.结果彝族高血压组与正常对照组DD、ID、II基因型频率分别为19.6%、46.9%、33.5%和17.1%、42.6%、40.3%,基因D、I频率分别为43.0%、57.0%和38.4%、61.6%,两组基因型频率分布和基因频率比较差异无统计学意义(P>0.05).彝族男性高血压组与正常男性对照组、彝族女性高血压组与正常女性对照组的基因型频率分布和基因频率比较差异无统计学意义(P>0.05).结论ACE基因可能不是彝族原发性高血压的主要遗传易感因素.更多还原

【Abstract】 Objective To investigate the relationship between ACE gene I/D polymorphism and essential hypertension in Yi people of Yunnan province.Methods We recruited 194 hypertensive and 129 normotensive subjects of Yi people in rural area of Jinning,Yunnan province.The ACE I/D polymorphism was determined by the polymerase chain reaction (PCR) technique.Frequencies for the DD,ID,II genotypes and allele frequencies for the D,I alleles were calculated.Then the possible association between the ACE I/D polymorphism and essential hypertension was analysed.Results Frequencies for the DD,ID and II geonotypes were 19.6%,46.9% and 33.5% in hypertensive subjects,and 17.1% ,42.6% and 40.3% in normotensive subjects,respectively.Derived allele frequencies for the D and I allele were 43.0% and 57.0% in hypertensive subjects and 38.4% and 61.6% in normotensive subjects.The distribution of ACE genotypes and the allele frequencies did not differ significantly between hypertensive and normotensive subjects.They also showed no significant difference between cases and controls in Yi males or in Yi females.Conclusion The I/D polymorphism of ACE is probably not involved in the pathogenesis of essential hypertension in Yi people.更多还原