【摘要】 目的研究低层流切应力作用下,血管内皮细胞表面保护性脂蛋白高密度脂蛋白的特异性受体B族Ⅰ型清道夫受体的表达水平,探讨切应力对内皮细胞表面B族Ⅰ型清道夫受体表达的影响在动脉粥样硬化发生中的可能作用。方法无菌条件下取健康产妇剖宫产分娩的胎儿新鲜脐带培养人脐静脉内皮细胞,选择2~4代的细胞用于实验,将内皮细胞种植于载玻片上培养,待细胞长满汇合后放入流室系统中。实验分为实验组和对照组,选择静态条件下未施加切应力组为对照组,实验组为低切应力组(4.2 dyne/cm2)。切应力加载时间为1 h、2 h、4 h和8 h。作用完成后行半定量RT-PCR测定B族Ⅰ型清道夫受体的表达。结果在层流低切应力(4.2 dyne/cm2)刺激后,B族Ⅰ型清道夫受体表达随时间延长明显减弱,在8 h达低谷,在电泳图上几乎不显现。结论高密度脂蛋白的特异性受体B族Ⅰ型清道夫受体在低切应力(4.2 dyne/cm2)作用下表达减弱;低切应力可能通过下调B族Ⅰ型清道夫受体的表达,降低胆固醇的逆转运,促进血管动脉粥样硬化的发生。更多还原

【Abstract】 Aim To research the expression level of scavenger receptor BⅠ(SR-BⅠ) in endothelial cells under low laminar shear stress,and to investigate the possible role of SR-BⅠ on the formation of atherosclerosis. Methods The fresh fetal umbilical cord were taken from the health parturient women,and the human umbilical vein endothelial cell(hUVEC) were cultured.The second to fourth generations of endothelial cells will be used in the experiment and then the endothelial cells were planted on the glass slide.After the cell covering the glass slide completely,the glass slide was put in the flow chamber system.The endothelial cells was dealt with different shear stress: the control group was not dealt with shear stress,the dealt group was dealt with the low shear stress(4.2 dyne/cm2).The two groups were treated for 1 h,2 h,4 h and 8 h.Taking the glass slide out of the flow chamber system,total RNA was extracted and the expression level of the SR-BⅠ was detected with RT-PCR and agargel electrophoretic analysis according to the operation instruction of the RT-PCR box. Results The expression of SR-BⅠ became weaker and weaker when it was stimulated continuously by the low shear stress(4.2 dyne/cm2),the lowest expression of SR-BⅠ was at the 8 h,and it almost didn’t appear in the electrophoresis picture. Conclusion The expression of SR-BⅠ decreased when it was treated by low shear stress.The harmful mechanism of the low shear stress is that it can reduce the anti-transfer of the cholesterin through decreasing the expression of the SR-BⅠ,and increase the incidence of atherosclerosis.更多还原