【摘要】 Wolbachia是一类广泛存在于节肢动物体内的共生菌。玉米螟赤眼蜂Trichogramma ostriniae是我国玉米田间的优势赤眼蜂种,据报道,赤眼蜂种内有Wolbachia感染。本文利用Wolbachia的16s rDNA和wsp基因引物通过PCR方法对玉米螟赤眼蜂的野生种群进行了调查,发现以wsp基因为鉴定依据,检测的所有个体都感染了3种Wolbachia[wOstGDAa(GenBank accession no.EU157103),wOstGDAb(GenBank accession no.EU157104)和wOstGDB(GenBankaccession no.EU157105)]。本文首次报道了野生赤眼蜂种群内Wolbachia的三重感染率几乎为100%。根据本研究的结果,可以推测当不同种赤眼蜂寄生同一寄主时,Wolbachia可能会在不同赤眼蜂种间进行横向传播。更多还原

【Abstract】 s:Wolbachia are common bacteria found in arthropods.Trichogramma ostriniae is the major Trichogramma species in maize fields in China and it has been reported that Trichogramma species harbored Wolbachia.In this study,the Wolbachia wsp and 16s rDNA gene sequences were used to detect the infection of Wolbachia in natural populations of T.ostriniae.The results indicated that T.ostriniae was triply infected with three strains of Wolbachia based on the wsp gene,i.e.,wOstGDAa(GenBank accession no.EU157103),wOstGDAb(GenBank accession no.EU157104)and wOstGDB(GenBank accession no.EU157105).Phylogenetic analyses showed that wOstGDAa and wOstGDAb belong to supergroup A,while wOstGDB belongs to supergroup B.An extensive survey of Wolbachia infection in natural populations of T.ostriniae revealed that nearly all individuals tested were infected with wOstGDAa,wOstGDAb and wOstGDB.This is the first report that nearly 100% of the individuals in the population were triply infected with Wolbachia.According to our results,we suppose that Wolbachia may transfer among different Trichogramma species when they share a host egg.更多还原