【摘要】 目的建立高效液相色谱-蒸发光散射检测法测定阿拉伯糖含量的方法。方法采用SHISEIDONH2分析色谱柱(4.6mm×250mm,5μm),柱温:30℃,流动相:乙腈-水(70:30),流速:0.8mL/min,ELSD参数:漂移管温度为80℃,N2流速为2.0L/min。结果阿拉伯糖在12.00～27.99μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为100.4%,RSD=0.8%,定量限为200μg/mL(S/N=10)。结论HPLC-ELSD法简便,快捷,无干扰,灵敏度高,重现性好,可用于阿拉伯糖的含量测定。更多还原

【Abstract】 Objective To establish a HPLC-ELSD method for the determination of arabinose.Methods The analytical column was SHISEIDO NH2(4.6 mm×250 mm,5 μm).The column temperature was 30℃.The mobile phase was acentonitrile-water(70:30).The flow rate was 0.8 mL/min.The samples were determined by an evaporative light-scattering detector(ELSD) with the drift tube temperature at 80 ℃ and the flow rate of nitrogen was 2.0 L/min.Results The linear range of arabinose was 12.00～27.99 μg(r=0.999 9).The average recovery was 100.4% with RSD of 0.8%.The limit of detection and quantification for arabinose was 200 μg/mL(S/N=10).Conclusion The method is simple, accurate, sensitive and with good reproducibility.It can be used as the content control of arabinose.更多还原