【摘要】 目的探讨永久性前脑缺血大鼠海马CA1区脑源性神经营养因子(BDNF)及其受体酪氨酸蛋白激酶B(TrkB)蛋白表达水平的改变。方法采用永久性结扎双侧颈总动脉的方法制造永久性前脑缺血大鼠模型。造模8周后对大鼠大脑石蜡切片进行免疫组织化学染色,测定海马CA1区BDNF以及TrkB的定位、相对定量表达。抽提大鼠海马CA1区组织蛋白,采用Western Blot对BDNF以及TrkB的蛋白表达水平进行定量分析。结果永久性前脑缺血8周后,大鼠海马CA1区BDNF免疫反应阳性产物主要分布于锥体细胞的胞浆、胞核及轴突;模型组大鼠的BDNF蛋白表达水平显著升高(P<0.05)。大鼠海马CA1区TrkB免疫反应阳性产物主要分布于神经元的胞浆及轴突内;与假手术组相比,模型组大鼠海马CA1区TrkB蛋白表达水平无显著改变(P>0.05)。结论双侧颈总动脉结扎造成的永久性前脑缺血可增加大鼠海马CA1区BDNF蛋白表达水平,而对其受体TrkB的蛋白表达水平无显著影响。更多还原

【Abstract】 Objective To investigate the changes in expression levels of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) receptor in hippocampal CA1 region of permanent forebrain ischemia rats.Methods Permanent occlusion of bilateral common carotid arteries were performed to establish the rat permanent forebrain ischemia model. 8 weeks after surgery rats were perfused intracardially. The protein levels of BDNF and TrkB in hippocampal CA1 subfield were probed with immunohistochemistry staining and western blot.Results The immunoreactive products of BDNF distributed in the cytoplasm,neucli and axons of pyramidal neurons within hippocampal CA1 subfield. The protein level of BDNF was higher in model group than in sham-operated group (P<0.05). The immunoreactive products of TrkB distributed in the cytoplasm and axons of pyramidal neurons within hippocampal CA1 subfield. But the protein level of TrkB didn’t change significantly in model rats,compared to sham-operated rats (P>0.05).Conclusion Permanent occlusion of bilateral common carotid arteries could induce the increase in BDNF expression level,but didn’t remarkably impact the TrkB expression.更多还原