【摘要】 目的阐明2000～2007年陕西省流行的麻疹野病毒分离株的基因特征,为控制并消除麻疹提供依据。方法2000～2007年采集陕西省麻疹暴发和散发患者的咽喉拭子标本147份,用EB病毒转化的狨猴淋巴母细胞(B95a)分离到麻疹病毒21株,通过逆转录-聚合酶链反应(RT-PCR),从分离到的麻疹病毒株中扩增出核蛋白基因羧基末端450个核苷酸片段,再对扩增产物进行核苷酸序列测定和基因分型。结果21株麻疹病毒均属H1基因型,其中H1a 18株,H1b 3株,H1a和H1b在450个核苷酸片段中的差异为2%～4%。结论H1a和H1b在陕西省的流行状况提示,流行优势株的变化可能受本土病毒变异和邻省输入病毒的双重影响;同时在陕西省存在同一毒株的持续循环。更多还原

【Abstract】 Objective To illustrate the genetic characterizations and molecular epidemiology of wild-type measles viruses circulated in Shanxi Province during 2000-2007,and provide the scientific evidence for measles control and elimination.Methods Vero-SLAM cell was selected for measles viral isolation,and total of 21 measles viruses isolated from 147 throat-swabs specimens collected from the patients with measles.Fragments of 450 nucleotide acids of the carboxyl end of mucleo-protein gene were amplified by using reverse transcription-polymerase chain reaction(RT-PCR) method,and then the PCR products were directly sequenced and analyzed.Results 21 measles viruses isolated were all identified as H1 genotype,18 measles viruses belonged to H1a subgeno type,and the remaining three viruses H1b subgeno type.The difference at 450 nucleotide fragment between H1a subgeno type and H1b subgeno type was 2%-4%.Conclusion The prevalence of H1a and H1b indicated that the change of predominant genotype of wild measles virus circulated in Shanxi Province may be effected by the variation of local virus or the viruses input from the neighbouring provinces.At the same time,circulation of the same measles strain existed for a long time in Shanxi province.更多还原