【摘要】 目的观察十全大补汤配方颗粒对骨髓抑制小鼠外周血及促红细胞生成素(EPO)的影响。方法采用60钴(60Co)照射、注射环磷酰胺、氯霉素复合处理建立小鼠骨髓抑制模型后,给予十全大补汤配方颗粒进行治疗,采用全自动血细胞分析仪检测其对外周血的影响;双抗体夹心生物素-亲和素复合物酶联免疫吸附试验(ABC-ELISA)法检测血清中EPO含量;逆转录-聚合酶链反应(RT-PCR)检测骨髓及肾脏EPO mRNA表达情况。结果与正常对照组比较,模型组小鼠外周血血象、骨髓有核细胞数降低,血清EPO含量升高,肾脏、骨髓细胞EPO mR-NA表达增强。经十全大补汤配方颗粒治疗后,外周血、骨髓有核细胞数升高,血清EPO含量升高,肾脏、骨髓有核细胞EPO mRNA的表达增强。结论十全大补汤配方颗粒可能通过在转录水平上促进肾脏、骨髓有核细胞EPOmRNA的表达,促进受损骨髓红系造血的恢复。更多还原

【Abstract】 Objective To examine the effect of Shi-Quan-Da-Bu-Granule on routine blood,EPO serum concentration and expression of EPOmRNA in kidney and BMC of myelosuppressed mice.Methods Set up myelosuppressed mice model by means of Co60 irradiation,cyclophosphamide injection,and chlorampHenicol injection.The count of blood cell was determined by blood auto-analyzer.Serum concentration of Epo was measured by ELISA.Expression of EPOmRNA in kidney and BMC were determined by RT-PCR.Results Compared with normal control group,the count of blood cell,the number of BMC decreased,and serum concentration of EPO and expression of EPOmRNA in kidney and BMC increased.All of above indexes in high-dose and mid-dose of Shi-Quan-Da-Bu-Granule treatment group were higher than that of model control group(P<0.05).Conclusion Shi-Quan-Da-Bu-Granule could promote erythroid hematopoiesis by enhancing the production of EPO and up-regulating expression of EPOmRNA in kidney and BMC of myelosuppression mice.更多还原