【摘要】 目的探讨替勃龙对去势大鼠脑缺血再灌注后IL-1β和TNF-α表达的影响。方法30只雌性成年SD大鼠,随机分为假手术组,手术对照组,替勃龙组。采用大鼠大脑中动脉闭塞制成局灶性脑缺血模型,在缺血2h再灌注24h后立即断头取脑,应用TTC染色观察脑梗死体积,免疫组化法检测IL-1β和TNF-α的表达,并进行组织病理学观察。结果替勃龙用药组较对照组脑梗死体积显著缩小(P<0.05);替勃龙组较对照组IL-1β及TNF-α表达减弱(P<0.01)。结论替勃龙可降低去势大鼠脑缺血再灌注后IL-1β、TNF-α水平,减轻脑缺血再灌注损伤。更多还原

【Abstract】 Objective To study the effect of tibolone on expression of IL-1β and TNF-α in ovariectomized rats during cerebral ischemia-reperfusion injury.Methods Thirty healthy adult female Sprague Dawley rats were selected and randomly divided into sham-operated group,ovariectomized group,tibolone group 2.5mg/(d·kg).A brain ischemia model was induced by left middle cerebral artery occlusion for 2 hours.All animals were killed 24 hours after reperfusion.Cerebral infarction volume was meausred with 2,3,5 triphenyltetrazlium chloride(TTC)staining and image analysis.The expression of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)was detected by immunohistochemistry.Changes in tissue pathology were also investigated.Results The cerebral infarction volume was significantly lower in tibolone group than in ovariectomized group(P<0.05).The expression of IL-1β and TNF-α was obviously lower in tibolone group than in ovariectomized group(P<0.01).Conclusion Tibolone decreases the expression of IL-1β and TNF-α,thus protecting brain tissue against cerebral ischemia-reperfusion injury.更多还原