【摘要】 采用固体培养和液体发酵相结合的方法,探索了培养真菌常用的7种碳源,10种氮源、7种无机盐和5种维生素对茶藨生柱锈重寄生菌拟盘多毛孢之一种(Pestalotiopsissp.)MM011菌株菌丝生长的影响。结果表明,多糖(可溶性淀粉、玉米粉)是该重寄生菌菌丝生长的最佳碳源;最佳氮源为有机氮中的黄豆粉、酵母浸膏和蛋白胨;最适无机盐为磷酸二氢钾和磷酸二氢钠;不同维生素对菌丝生长有一定影响,但作用不明显。更多还原

【Abstract】 The MM011(Pestalotiopsis sp.)— mycoparasite of Cronartium ribicola was cultured in solid and liquid culture media using 7 hydrocarbons,10 nitrogenous compounds,7 inorganic salts and 5 vitamins.It was found that polysaccharides(solvable starch and maize flour) were favorable for mycelial growth,organic nitrogen(protein peptone,yeast extracts,soy powder) was the optional nitrogen source,inorganic salts(sodium phosphate and potassium phosphate) were more favorable for mycelial growth than others,mycelial growth varied in different growth factors,but the facilitation wasn’t obvious.更多还原