【摘要】 目的探讨外周血中鸟苷环化酶CmRNA(GCC-mRNA)的表达对大肠癌微转移的诊断意义。方法1、制备微转移模型;2、收集周围血标本:大肠癌患者67例,正常人及良性胃肠道疾病患者20例。3、检测GCC-mRNA:逆转录聚合酶链反应法检测GCC-mRNA。结果1、微转移模型:微转移模型GCC-mRNA表达阳性率为85%;对照组正常人外周血20例中无一例阳性;大肠癌微转移模型标本及对照组中的表达差异显著(P<0.001)。2、结肠癌:结肠癌GCC-mRNA表达阳性率为58%,良性胃肠道疾病为20%,正常人为0%,三组比较差异显著(P<0.01)。大肠癌患者有结肠外转移阳性率为72%;无转移阳性率为52%,二组差异无显著性(P>0.05)。3、GCC与CEA:GC-C与CEA总阳性率分别为58%与27%,前者显著高于后者(P<0.01)。结论1、提示RT-PCR法检测GC-C诊断大肠癌微转移方法有希望用于诊断大肠癌微转移。2、提示GC-C有希望用于结肠癌患者微转移的诊断指标,且优于CEA。更多还原

【Abstract】 Objectine To explore the relation between guanylate cyclase C and colorectal cancer recurrence or metastasis.Methods Micro-metastases models of colorectal cancer were made by mixture of colon cancer cells with blood of healthy population.Reverse transcription-polymerase chain reaction(RT-PCR) was employed to test the expression of GCC-mRNA.Results The expression rate of GCC-mRNA was significantly higher in the micro-metastases models than that of healthy persons(P<0.01).The expression rate of GCC-mRNA was significantly higher in the colon cancer patients than that of benign disease in gastroenterology(P<0.01).There was no significant difference of GCC mRNA expression rate between the metastases of clorectal cancer and the nonmetastases of clorectal cancer(P>0.05).Conclusion The GC-C-positive rate is higher than the CEA expression in patients with colorectal cancer, so GC-C detection is hope for the diagnosis of the metastases of clorectal cancer.更多还原