Objective:To clone and identify the expressed location of human antiapoptotic gene-Survivin in hepatic carcinoma.Methods:Survivin cDNA was obtained by using RT-PCR method with total RNA extracted from the human hepatic carcinoma,and it was subcloned into eukaryotic expression vector pEGFP-N2,and then pEGFP-Survivin was obtained.After transiently transfecting the human liver cancer cell SMMC-7721 with the recon contained Survivin fragment,confocal microscopy was applied to observe the location of transfected...
【更新日期】
2009-10-16
【分类号】
R735.7
【正文快照】
肝癌是全球发病率最高的恶性肿瘤之一,其中约55%发生在中国[1],至今仍缺乏有效的治疗手段。Survivin属于凋亡抑制蛋白(inhibitor of apoptosisfamily of protein,IAPS)家族的成员之一,是目前最强的凋亡抑制因子,具有抑制细胞凋亡,促进细胞转化的功能。为了研究Survivin基因在肝
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