【摘要】 目的:观察日粮添加核苷酸对CCl₄染毒大鼠肝纤维化形成的干预作用.方法:选用90只SD大鼠随机分为空白对照组,四氯化碳模型组和四氯化碳+核苷酸组.四氯化碳模型组和四氯化碳+核苷酸组大鼠腹腔注射四氯化碳,对照组大鼠注射橄榄油,每周2次,连续12周.对照组和四氯化碳组饲喂大鼠纯化饲料,四氯化碳+核苷酸组饲喂添加有核苷酸的纯化饲料.结果:在试验期内,四氯化碳组大鼠体重增长低于或显著低于对照组,其中第2、4、6、8、10周时差异显著,四氯化碳+核苷酸组大鼠体重增长虽低于对照组,但无显著差异;在整个试验期内大鼠的死亡率,四氯化碳组为20%,而四氯化碳+核苷酸组仅为7%;四氯化碳模型组大鼠肝脏组织MDA含量较空白对照组和四氯化碳+核苷酸组高;从肝脏外部形态和色泽看,四氯化碳组表面油腻,有结节,四氯化碳+核苷酸组表面光滑.结论:日粮添加核苷酸对CCl₄染毒致大鼠肝纤维化具有一定干预作用.更多还原

【Abstract】 Objective Effects of dietary nucleotides on hepatic fibrosis induced by CCl₄ were studied.Methods 90 SD rats were divided randomly into a control group,a CCl₄ group and a CCl₄ + NTs group.Rats in CCl₄ group and CCl₄ + NTs group got abdominal injection of CCl₄ 2 times a week for 12 weeks, the CCl₄ + NTs group was fed with a nucleotide-supplemented diet,the control group and the CCl₄ group were fed with a nucleotide-free diet.Results The survival rate of CCl₄ + NTs group rats is 93%and the CCl₄ group is only 80%.The rats weight of the CCl₄ group is lighter than the CCl₄ + NTs group,and markedly in the 2nd,4th,6th,8th and 10th week.The rats weight of the CCl₄ + NTs group is also lighter than the control group,but not markedly.In the CCl₄ group,the content of MDA in liver homogenate higher than the CCl₄ + NTs group and control group.And the morphologic features of rat liver shows the NTs group have a milder liver injury.Conclusion Dietary Nucleotides may interfere the hepatic fibrosis induced by CCl₄ in rat.更多还原