【摘要】 目的:研究利用微生物微孔板培养及三维图形显示技术建立一种基于微生物(大肠杆菌)生长状态估测电磁辐射场强空间分布方法的可行性。方法:按照常规分子克隆方法将转化入质粒pET28b-tat-EGFP(Kana+抗性,卡那霉素抗性)的大肠杆菌BL21(DE3)接种到96孔板(150μl/孔),置于工作频率为34.1GHz、平均功率密度为12W/cm2的高功率毫米波辐射源正下方分别连续照射1、5和10min,照射后迅速置于37℃恒温箱培养6h,检测D570值并在MatLab软件中采用三次方程插值法实现所测微孔板数据的三维图形显示,进而与红外热像仪监测结果及计算机模拟计算结果相比较,从而估计特定空间辐照强度的分布情况。结果:毫米波辐照1～5min后,微孔板中靠近边缘区域的部分菌体生长增强,表现为三维图形中的波峰,而中间区域的菌体生长状态基本不变或被轻度抑制;照射10min后,微孔板中心区域的菌体生长状态受到明显抑制,表现为三维图形中的波谷,而周边微孔中的菌体生长状态基本不受影响,且此时红外热像仪监测结果显示微孔板中心区域最高温度达52.4℃。利用MatLab进行三维图形模拟显示的结果与红外热像仪监测结果及计算机模拟计算结果基本吻合。结论:利用微生物(大肠杆菌)的生长状态间接估测毫米波辐射源场强空间分布的方法是可行的,为进一步深入研究电磁辐射的生物效应奠定了基础,可望为电磁辐射的生物剂量学研究提供新的参考手段。更多还原

【Abstract】 Objective:To evaluate the feasibility of a method for estimation of the spatial distribution of the electromagnetic field based on the growth state analysis of microbiology using microplate technique and graphics display technique. Methods:The strain E.coli BL21 (DE3),transformed with the vector pET28b-tat-EGFP (Kana+),was inoculated in the 96-well microplate and exposed to millimeter wave of 34.1 GHz with mean power density of 12W/cm2 for 1 min,5 min and 10 min,respectively. The E.coli BL21 (DE3) cells were then incubated in the 37℃-incubator for 6 h followed by the optical density measurement at 570 nm. The data were displayed as three-dimensional graphics using the interpolation method in MatLab,and then compared to the traditional computer simulation and infrared monitoring methods.Results:The growth of bacterial cells was promoted when exposed to millimeter wave for 1-5 min,with peaks displayed in the 3D simulation graph using MatLab. However,the growth of most bacterial cells was inhibited when exposed to millimeter wave for 10 min,showing troughs while the maximal temperature in the central region of the microplate was 52.4℃ monitored by the infrared thermal imager. The 3D simulation graphs were entirely consistent with the results of theoretical calculation and infrared thermal imager monitoring analysis.Conclusion:A method for estimation of the spatial distribution of the electromagnetic radiation field based on the growth state of microorganisms using microplate technique and graphical display approach is established in this paper,which is important for further and systematical study of the biological effects of electromagnetic radiation.更多还原