【摘要】 建立祖师麻的有效成分——祖师麻甲素提取和HPLC检测方法,并对其进行了量子化学研究。HPLC检测方法选用VP ODS C₁₈（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.5%磷酸溶液（45:55）,检测波长327 nm,量子化学计算在B3LYP/6-311G（2DF,PD）水平得到祖师麻甲素的几何构型、振动频率及能量,认识祖师麻甲素的分子内氢键结构。HPLC检测方法精密度、稳定性、重现性好,提取祖师麻甲素纯度为96.6%,量子化学计算结果与已知实验数据一致。更多还原

【Abstract】 The extracing process for daphnetin from zushima is studied and an HPLC method is developed for the detection of daphnetin in zushima.The analysis was carried out on a VP ODS C₁₈（250 mm×4.6 mm,5μm） column.The mobile phase was methanol -0.5%phosphoric acid（45:55） and detection wavelength was 327 nm.Quantum chemistry calculation was performed at B3LYP/6- 311G（2DF,PD）level.The geometry structures,vibrational frequency and energies of daphnetin were discussed,some property shows that there is intramolecular hydrogen-bonding in this compound.更多还原