For the induction of a good root system of the shoot of Daphen odora var.marginata,in vitro,it was in need of the composition of NAA and IBA or NAA and IAA,besides it was most important to add enough amount of sucrose for increase the rooting rate,the number of root per shoot generated and the length of root.Through the comparison between 6 kinds of rooting mediums,1/2MS+NAA 0.5 mg/L+IBA 0.5 mg/L+3%sucrose was determined as the best one and a rooting ate of 84.6% was obtained in this medium.