【摘要】 目的探讨内皮抑素在兔VX2肝移植瘤介入治疗中的应用价值。方法建立兔肝移植瘤模型,随机分为对照组(NS,10只),TACE组(Lipiodol+ADM,10只)和内皮抑素组(Lipiodol+ADM+ES,10只),多排螺旋CT测量肿瘤大小,计算肿瘤增长率(GR);术后1周取出病理标本,利用免疫组化染色及半定量RT-PCR法分别检测残瘤组织微血管密度(MVD)和血管内皮生长因子(VEGF)表达。结果3组肿瘤增长率分别为对照组(270.86±148.94)%,TACE组(-8.91±21.77)%,内皮抑素组(-20.40±36.07)%,3组间相互比较,对照组呈明显正增长,与其余2组比较差异均有统计学意义(P<0.01),而TACE组与内皮抑素组之间差异无统计学意义(P>0.05);各组的MVD值分别为80.00±17.14(对照组)、84.22±16.45(TACE组)和57.00±13.26(内皮抑素组),内皮抑素组MVD值最低,与另外2组比较差异均有统计学意义(P<0.05),对照组和TACE组之间差异性不明显(P>0.05);VEGF165mRNA:对照组为(0.85±0.056),TACE组为(1.10±0.087),内皮抑素组为(0.72±0.065),TACE组表达水平最高,与其余2组比较差异有统计学意义(P<0.01)。结论兔VX2肝移植瘤的介入治疗中应用内皮抑素后,肿瘤明显缩小,肿瘤组织VEGF的表达减低,MVD的分布减少,提高了肿瘤的治疗效果。更多还原

【Abstract】 Objective To investigate the clinical application of angiogenesis inhibitor, endostatin, in interventional treatment for hepatic tumor by means of experimental study in rabbits with VX2 hepatic implantation tumor. Methods According to the method introduced in academic documents, the animal model of VX2 hepatic implantation tumor was established in 30 rabbits. The rabbits were equally and randomly divided into three groups, with 10 rabbits in each group. Group A, regarded as control group, was treated with normal saline. Group B, regarded as TACE group, was treated with transcatheter arterial chemoembolization (lipiodol + ADM) only. Group C (anti-angiogenic group) was treated with chemoembolization (lipiodol + ADM) combined with endostatin. Enhanced multi-slice CT scanning was performed before and after the procedure as well as before sacrifice of the rabbits. The tumor size and growth rate was measured and calculated. Seven days after the treatment, the rabbits were sacrificed and the tumor specimens were collected and sent for exams. Using immunohistochemical staning and RT-PCR technique, the expression of microvessel density (MVD) and vascular endothelial growth factor (VEGF) in the residual tumor were determined separately. Results The tumor growth rate in group A, B and C was (270.86 ± 148.94)%, (-8.91 ± 21.77)% and (-20.40 ± 36.07)%, respectively. In comparison, after the treatment the tumor size in group A showed a marked positive enlargement. Significant difference in tumor growth rate existed between group A and other two groups (P < 0.01), while no significant difference in tumor growth rate existed between group B and group C (P > 0.05). The MVD in group A, B and C was 80.00 ± 17.14, 84.22 ± 16.45 and 57.00 ± 13.26, respectively. The MVD in group C was the smallest, and there was a striking difference (P < 0.05) between group C and other two groups, while there was no remarkable difference (P > 0.05) between group A and group B. As for the expression of VEGF, the relative light intensity of the group B was 1.10 ± 0.087, which was significantly higher than that of group A (0.85 ± 0.056) and of group C (0.72 ± 0.065), with P < 0.01. Conclusion For the interventional treatment of VX2 hepatic implantation tumor in rabbits, chemoembolization combined with endostatin is superior to simple TACE. It can depress the expression of VEGF, inhibit the angiogenesis of the tumor and reduce the tumor size, which are helpful to improve the therapeutic effect.更多还原