【摘要】 目的探讨牦牛蹄缘不同位点毛囊形态发生的规律及音猬因子(SHH)和β-连锁蛋白(-βcatenin)的时空表达。方法选择41例胚龄(E)45~248d牦牛蹄缘样品,采用石蜡组织切片、HE染色对蹄缘不同位点毛囊的形态发生过程进行观察分析;用EnVison免疫组织化学法检测SHH和-βcatenin的时空表达。结果牦牛蹄缘近蹄端毛囊的形态发生约开始于E45d,远蹄端为E55d,形成成熟毛囊的时间约为E120d;牦牛蹄缘近蹄端毛囊的形态发生先于远蹄端,由近蹄端至远蹄端毛囊的发育阶段、毛囊大小和下陷深度呈梯度由近至远依次递减;SHH和β-catenin在牦牛蹄缘毛囊表达的时间顺序近蹄端先于远蹄端;在牦牛蹄缘毛囊形态发生过程中,SHH和-βcatenin在近蹄端和远蹄端毛囊表皮的表达强度均大于间充质;-βcatenin在近蹄端间充质中的表达强度大于远蹄端。结论SHH和-βcatenin在牦牛蹄缘毛囊形态发生的表皮-间充质相互作用中,以表皮为主导;不同位点毛囊形态发生的梯度是由SHH和-βcatenin在表皮与间充质中的特殊作用和时空表达规律决定的。更多还原

【Abstract】 Objective To investigate hair follicle morphogenesis of different hoof periphery site,SHH and β-catenin temprocal-spatial expression during hair follicle morphogenesis. Methods Totally 41 embryonic 45-248 days Yak embryos forepaw were used as material to investigate hair follicle morphogenesis of different hoof periphery site by HE staining and immunohistochemistry of tissue section for detecting temprocal-spatial expression and epithelial-mesenchymal reciprocal interaction during morphogenesis.SHH expression during hair follicle morphogenesis showed stronger effect on the hair follicle epidermis than the hair follicle mesenchyme. Results The hair follicle morphogenesis of yak proximal hoof end and distal hoof end initiate from embryonic 45 days and initiate from embryonic 55 days respectively,mature hair follicle in embryonic 120 days.From the proximal hoof end to distal hoof end,the time,the phase,the size and the growing depth of the hair follicle morphogenesis in turn showed a occure degressive gradient.β-catenin expression during hair follicle morphogenesis showed a stronger effect on the hair follicle epidermis than the hair follicle mesenchyme.Conclusion We speculate that SHH and β-catenin expression and detail function difference play important roles during hair follicle morphogenesis in different hoof periphery site of yak embryos.The epidermis is dominated during the hair follicle morphogenesis.更多还原