【摘要】 目的观察白假丝酵母菌生物膜形成过程中耐药性产生的变化情况。方法利用载玻片法形成白假丝酵母菌生物膜结构,倒置显微镜观察不同阶段的生物膜形态,用美国临床实验室标准化委员会(NCCLS)微量稀释法检测浮游状态白假丝酵母菌耐药性,用二甲氧唑黄(XTT)减低法获得生物膜耐药性的变化情况。结果白假丝酵母菌耐药性随着生物膜的成熟不断增强,比游离态菌高100倍以上。结论成熟生物膜与耐药性增强有明显正相关性。更多还原

【Abstract】 Objective To observe the drug resistance at different stages of Candida(C.) albicans biofilm formation.Methods To form the biofilm in glass slide,then observe the biofilm morphology by inverted microscope at different development stages(2,4,8,24,48 hours).Using National Committee for Clinical Laboratory Standards(NCCLs) microdilution to detect drug resistance of dissociative C.albicans and 2,3 bis(2methoxy 4 nitro 5 sulfophenyl) 5(phenylamino) carbonyl 2H tetrazolium hydroxide(XTT) reduction method to test the change of C.albicans drug resistance in biofilm.Results Antifungal resistance of biofilm-grown cells increased as the biofilm maturation.Compared with dissociative C.albicans strains,the drug resistance was stronger more than 100 times.Conclusion There is positive correlation between drug resistance and biofilm maturation of C.albicans.更多还原