【摘要】 目的观察血管紧张素Ⅱ(AngⅡ)对体外培养孕12d小鼠肾组织中各期肾小体细胞增殖的影响。方法孕12d母鼠随机分为体外培养对照组和施加不同浓度AngⅡ(10-9mol/L～10-5mol/L)组。应用体外培养、免疫组织化学及免疫荧光技术,观察各组肾组织培养2、4、6d后各期肾小体增殖细胞核抗原(PCNA)的表达。结果AngⅡ能促进小鼠肾小体细胞的增殖,在一定范围内,AngⅡ浓度越大肾小体细胞增殖越明显,且与作用时间呈正相关;培养4d的肾组织比培养2d的肾组织增殖明显。结论AngⅡ对发育期小鼠肾小体细胞有促增殖作用,且此作用有浓度时间依赖性。更多还原

【Abstract】 Objective To observe the in vitro effects of angiotensin Ⅱ on cell proliferation of the renal corpuscle in embryonic day 12 mouse kidneys. Methods Pregant female mice(embryonic day 12)were randomly divided into six groups:control group and groups which were induced by different angiotensin Ⅱ(10-9 mol/L-10-5 mol/L) concentrations. The kidney tissues were cultured for two,four or six days.The proliferating cell nuclear antigen (PCNA) expression was detected by immunohistochemistry and immunofluorescence techniques. Results AngiotensinⅡ promoted cell proliferation of the renal corpuscle. To some extent,the proliferative activity tended to enhance with increasing of the angiotensinⅡ concentration,and the effect had a positive relation to induced time. The proliferative activity at the 4th day was stronger than that at the 2nd day.Conclusion AngiotensinⅡ can promote cell proliferation in the developing mouse renal corpuscle with dose-dependence and time-dependence effects.更多还原