【摘要】 目的探讨音猬因子(SHH)对神经干细胞(NSCs)增殖与分化的影响。方法从大鼠胚胎端脑皮质及海马分离培养的NSCs分为SHH组、RA组和PBS组。分别用SHH、维甲酸(RA)及PBS条件培养液处理后,通过免疫细胞化学等方法检测NSCs的增殖及分化情况。结果体外培养的NSCs能快速增殖并形成神经球,其中的细胞均表达NSCs特异性标志物nestin。BrdU作用于NSCs3h后,SHH组的BrdU阳性率明显高于RA组和PBS组,而RA组和PBS组之间无显著差异。当NSCs在分化条件培养液中培养7d后,SHH组和RA组中的βⅢ-tubulin阳性率及Rip阳性率显著高于PBS组,SHH组又显著高于RA组和PBS组。结论SHH可以促进NSCs增殖及其向神经元和少突胶质细胞的分化。更多还原

【Abstract】 Objective To explore the effect of sonic hedgehog on the proliferation and differentiation of neural stem cells (NSCs). Methods NSCs isolated from the cortex and hippocampus of rat embryos. were divided into SHH group,RA group and PBS group,which were treated with sonic hedgehog (SHH),retinoic acid (RA) or PBS condition medium respectively. Effect of these treatments on the proliferation and differentiation of the NSCs were detected by immunocytochemistry. Results NSCs could grow quickly and form neurospheres in culture. The cells in the neurospheres were positive for nestin which is one of the NSC markers. After the medium was supplemented with BrdU for 3h,the percentage of BrdU positive cells in the SHH group was significantly higher than that of the RA group and PBS group. However,there was no obvious difference between RA group and PBS group. After the NSCs were cultured in differentiating condition medium for 7 days,the percentages of βⅢ-tubulin and Rip positive cells in the SHH treated group were significantly higher than that in the RA and PBS treated groups. Conclusions SHH could promote NSC proliferation and differentiation into neurons and oligodendrocytes.更多还原