【摘要】 目的研究白细胞介素2(IL-2)致痫与蛋白酪氨酸激酶(Jak)/信号转导子和转录激活子(Stats)信号转导途径的关系。方法用免疫细胞化学方法研究IL-2致痫大鼠脑内蛋白酪氨酸激酶Jak1的表达变化,及预先应用Jak1抑制剂和糖皮质激素干预后,再用IL-2致痫后脑内N-甲基-D-天门冬氨酸型受体-1(NMDAR-1)和谷氨酸(Glu)的表达变化。结果侧脑室注射IL-2后大鼠出现明显的癫痫发作,其Jak1的表达较对照组明显增强(P<0.01),免疫抑制剂环孢霉素可部分抑制此效应;Jak1抑制剂植物异黄酮和免疫抑制剂糖皮质激素可明显抑制IL-2致痫大鼠的癫痫行为,其NMDAR-1和Glu的表达较IL-2组显著降低(P<0.05)。结论在IL-2致痫中,Jak1在其信号转导途径中发挥重要作用。更多还原

【Abstract】 Objective To explore the relationship of the epilepsy induced by interleukin-2(IL-2) and the Janus kinase(Jak)/ signal transducers and activators of transcription(Stats)signal transduction pathway.Methods Immunocytochemistry was applied to study the expression changes of tyrosine kinase Jak1 in brain after IL-2 administration to induce epilepsy,and the changes of N-methyl-Daspartate receptor-1(NMDAR-1) and glutamic acid(Glu) when preusing Jak1 inhibitor and glucocorticoid prior to IL-2.Results After injecting IL-2 into the lateral ventricle,the rats displayed obvious seizures and the expression of Jak1 was significantly stronger comparing with the control group(P<0.01),immunosuppressants such as cyclosporin A(CsA) could partly inhibit this effect.Jak1 inhibitor genistein and immunosuppressant glucocorticoid could restrain the epilepsy behaviors,and the NMDAR-1 and Glu expressions significantly decreased conferring to the IL-2 group(P<0.05).Conclusion Jak1 plays an important role in signal transduction of IL-2 in epileptogenic mechanisms.更多还原