【摘要】 目的:探讨末梢全血和静脉血待测时间对白细胞及血小板计数的影响。方法:33例末梢全血标本和20例静脉血标本被分别注入EDTA-K2抗凝管,轻轻充分混匀后立即在血细胞分析仪检测,然后再分别放置10min和30min后,再次混匀测定,对末梢全血与静脉血在三个时间测定的白细胞(WBC)、血小板(PLT)结果进行比较分析。结果:末梢全血10min的结果与即测的结果比较:白细胞数明显降低(P<0.01),血小板数明显升高(P<0.001);30min结果与10min结果比较,差异无统计学意义(P>0.05)。静脉血各个时间的测定结果间均差异无统计学意义(P>0.05)。结论:末梢全血采集过程中由于组织液混入,促使血小板短时间内产生一个可逆的假性聚集,从而影响WBC和PLT结果的准确性;末梢全血采集后放置10min后再测可提高检测的准确性;静脉血采集混匀后即上机检测对结果影响不大。更多还原

【Abstract】 Objective To study the waiting time of periphery blood and venous blood on counting of WBC and PLT.Method Thirty-three cases periphery blood and 20 cases venous blood were injected into anticoagulation tube(EDTA-K2),and they were determined after mixed immediately,then were determined 10 min and 30 min later.WBC and PLT count were contrasted among 3 different times.Results The periphery blood determine results:10 min later vs.immediately:WBC count was significantly lower(P<0.01),PLT count was significantly higher(P<0.001);30 min vs.10 min:WBC and PLT count had no significant difference(P>0.05).The venous blood determine results at different times had no significant difference(P>0.05).Conclusion Periphery blood mixs with tissue fluid when it is gathered,and platelet shows some reversible pseudo accumulation,then influences the accuracy of WBC and PLT count/periphery blood is determined 10 min later can improve the accuracy.Venous blood is determined immediately after mixed,can not affect the results significantly.更多还原