【摘要】 以生物可降解材料PLG607为载体,采用S/W/O/W溶剂挥发法制备牛血清白蛋白(BSA)微球,对其性质进行表征,并考察了处方中不同添加剂对BSA-PLG微球粒径、包封率和体外释放的影响.结果表明,采用S/W/O/W法所得微球粒径为40~70μm,表面光滑圆整.加入添加剂(m(羟丙基-β-环糊精)∶m(牛血清白蛋白)=1∶1)后包封率可以从27.9%提高到46.3%,加入添加剂羟丙基-β-环糊精和羟基磷灰石制备蛋白微球,能减小突释,提高蛋白的包封率,改善体外释放行为,达到均匀释放.更多还原

【Abstract】 The BSA-PLG microspheres were prepared by S/W/O/W emulsion solvent evaporation method with biodegradable material PLG607 as the carrier.Drug encapsulation capacity,morphology,particle size characteristics,as well as in-vitro release profiles of the microspheres were discussed.The results show that the microspheres were spherical and smooth and the mean size of the microspheres was 40~70 μm,with a mono dispersibility.The in-vitro release profiles of the microspheres produced from different methods were significantly distinct.With the additives of hydroxypropyl-β-cyclodextrin(HPB) and hydroxyapatite(HAP),the encapsulation of microspheres increased from 27.9% to 46.3%.Therefore,using S/W/O/W method and adding additives HAP and HPB in the formulation could decrease the initial burst release of drugs from the microspheres and increase the encapsulation capacity of the BSA-PLG microspheres,the in-vitro release profiles of which were fitted into near zero order release kinetics.更多还原