【摘要】 对已经构建成功并转化到大肠杆菌E.coliBL21(DE3)的基质金属蛋白酶MT5-MMP的催化结构域进行诱导表达,得到分子量为21 000,包涵体形式的重组蛋白.通过离子交换树脂对目的蛋白进行纯化后,用凝胶过滤树脂对纯化后的蛋白进行柱上复性,得到了具有较高活性的重组蛋白.更多还原

【Abstract】 The authors completed the induction expression of recombinant MT5-MMP in E.coli BL21(DE3) and harvested the protein as inclusion bodies.The molecular weight of the protein is about 21 000.In addition,we purified and refolded the recombinant protein using the chromatography method to obtain the catalytically active MT5-MMP.更多还原