【摘要】 目的探讨淋巴毒素-β(LT-β)在狼疮性肾炎发病及免疫调节紊乱中的作用。方法用ELISA检测血清LT-β水平,PBL RNA斑点杂交测定LT-β基因转录水平;PCR-RLFP法分析LT-β基因多态性。结果狼疮性肾炎患者血清LT-β水平比正常显著增高(P<0.01),与未合并狼疮性肾炎组比亦显著增高(P<0.01);基因转录水平比正常对照组显著增高(P<0.05),与未合并狼疮性肾炎组比亦显著增高(P<0.05)。未发现LT-β基因的XhoⅠ酶切位点具有多态性。结论提示LT-β是导致狼疮性肾炎发生的主要因子,LT-β参与SLE的免疫调节紊乱。更多还原

【Abstract】 Objective To study the effect of LT-βon inordinate immunoregulation in lupus nephritis patients.Methods The level of LT-βprotein in patients seurm were detected with ELISA.The level of LT-βgene transcription were detected with hybridiztion.The polymorphism of LT-βgene were investigated by PCR-RFLP analysis.Results The level of LT-βprotein showed significant difference between lupus nephritis patients and control groups(P<0.01);The level of LT-βprotein showed significant difference between lupus nephritis patients and non lupus nephritis patients groups(P<0.01).Hybridization by using LT-β DNA probe showed significant difference between lupus nephritis patients and control groups(P<0.05);Significant difference between lupus nephritis patients and non lupus nephritis groups was also showed(P<0.05).In both groups,the PCR products of LT-βgene 240 bp fragment were digested with XhoⅠ.Conclusion The results suggested the LT-βwas the important cytokine to conduct lupus nephritis and involve in the inordinate immunoreglution in SLE.更多还原