【摘要】 目的:根据机体对巨细胞病毒重组抗原pp52和pp150不同的IgG免疫反应,建立一种快速、简便和适用的ELISA方法,用于区分孕妇巨细胞病毒近期原发与非原发感染。方法:应用间接ELISA方法测定巨细胞病毒两种重组抗原pp52和pp150 IgG,计算pp52 OD与pp150 OD的比值(pp52 OD/pp150 OD)。结果:共检测858份血清,其中812例为既往感染,46例为活动性感染(15例为原发感染、31例为继发或再感染)。当以pp52 OD/pp150 OD比值>1.5作为原发感染标准时,4组的阳性率分别为1.5%、32.6%、66.7%和16.1%,各组间差异有统计学意义(P<0.05),方法的敏感性和特异性分别为66.7%和98%。结论:本研究建立的测定巨细胞病毒重组蛋白pp52 OD/pp150 OD的酶联免疫吸附方法是辅助区分巨细胞病毒原发与非原发感染的较好的血清学方法,有待扩大样本量进一步证实。更多还原

【Abstract】 Objective: To establish a rapid,easy and convenient enzyme linked immunosorbent assay(ELISA) method for distinguishing recent primary cytomegalovirus(CMV) infection from non-primary infection(past and secondary infection,reinfection or persistence infection) during pregnancy,according to the different IgG immune responses for CMV recombinant antigens pp52 and pp150.Methods: The CMV-IgG of recombinant antigens pp52 and pp150 was detected using indirect ELISA,and the ratio of pp52 OD and pp150 OD was calculated.Results: 858 serum samples were tested,812 cases among them were identified as past infection,and 46 cases of reactive infection(15 cases of primary infection and 31 cases of secondary infection or reinfection).When pp52 OD/pp150 OD>1.5 was defined as positive for recent primary CMV infection,the positive rates of four groups were 1.5%,32.6%,66.7% and 16.1%,respectively.There were statistical differences among each group(P<0.05).The sensitivity and specificity of ELISA method were 66.7% and 98%,respectively.Conclusion: The ELISA method,with the detection of pp52 OD/pp150 OD,established in this study can be applied as an assisted serological method for distinguishing primary CMV infection from non-primary infection.And further confirmation should be obtained by the expansion of sample sizes.更多还原