【摘要】 目的:观察男性不育症患者精浆中白细胞介素-1β(IL-1β)、白细胞介素-8(IL-8)、肿瘤坏死因子α(TNF-α)含量与精子部分功能指标关系。方法:应用放射免疫分析技术对118例男性不育症和22例正常生育者精浆中IL-1β、IL-8、TNF-α含量进行检测并分析。根据精子密度将不育症患者分为A组(精子密度≥20×106/ml)、B组(精子密度<20×106/ml)和C组(无精子症者)3组;根据精子活力、活率将A组分别分为精子活力正常组和不良组,精子活率正常组和下降组;根据精液中白细胞(WBC)数量将不育症患者分为WBC精液组和非WBC精液组。结果:不育症组精浆IL-1β、IL-8、TNF-α含量显著高于生育组(P<0.05或P<0.01);不育症组精浆中IL-1β含量在精子活力正常与不良、WBC精液组与非WBC精液组之间存在统计学差异(P<0.05);IL-8含量在精子活率正常与减少、WBC精液组与非WBC精液组之间有统计学差异(P<0.05或P<0.01);TNF-α含量在不育症组精子活力、活率正常与不良之间均有统计学差异(P<0.05)。结论:精浆中IL-1β、IL-8、TNF-α含量与男性生殖密切相关,其含量变化可反映生殖系统局部的免疫状态和感染情况,并影响精子的运动功能,含量测定可为不育症诊治提供参考。更多还原

【Abstract】 Objective: To determine the contents of seminal plasma interleukin-1β(IL-1β),interleukin-8(IL-8) and tumor necrosis factor-alpha(TNF-α) in patients with male infertility,and analyze the relationship between them and sperm function indexes.Methods: The contents of seminal plasma IL-1β,IL-8 and TNF-α of 118 patients with male infertility and 22 normal males were detected and analyzed using radioimmunoassay(RIA).All patients were divided into 3 groups according to the sperm counts: Group A(sperm counts ≥ 20×106/ml),Group B(sperm counts < 20×106/ml) and Group C(azoospermia).And group A was subdivided into the normal sperm vitality group and the abnormal group,the normal sperm motility group and the degression group based on sperm vitality and motility.The patients were divided into the WBC semen group and the non-WBC semen group in line with the amounts of white blood cells(WBC).Results: The contents of seminal plasma IL-1β,IL-8 and TNF-α in the infertility group were obviously higher than that of the normal group(P<0.05 or P<0.01).There were significant differences of seminal plasma IL-1β contents between the normal sperm vitality group and the abnormal group,as well as the WBC semen group and the non-WBC semen group(P<0.05).Significant differences of IL-8 contents were existed between the normal sperm motility group and the degression group,as well as the WBC and non-WBC semen groups(P<0.05 or P<0.01).And the differences of TNF-α contents were statistical significant(P<0.05),among the infertility group,normal sperm vitality and abnormal groups,as well as the normal sperm motility and degression groups.Conclusion: The contents of seminal plasma IL-1β,IL-8 and TNF-α are closely related to male reproduction.And the changes of their contents will reflect the state of topical immunity and infection of reproductive system,and influence the sperm functions.The determination of these factor contents will provide evidences for the diagnosis and treatment of male infertility.更多还原