【摘要】 目的:观察莲心碱对大鼠局灶性脑缺血/再灌注损伤后脑组织一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的影响,为莲心碱治疗脑缺血提供实验依据。方法:线栓法阻塞大鼠大脑中动脉2h/再灌注24h制备大鼠脑缺血再灌注损伤动物模型。24只SD雄性大鼠随机分为3组:假手术组、缺血再灌注组和莲心碱(3 mg/kg)组,分别于术前30 min、再灌注即刻、再灌注12h舌静脉注射莲心碱3 mg/kg。再灌注24h后处死大鼠,取脑组织以硝酸还原酶法测定脑组织中的NO水平,以化学比色法测定总NOS活性,并测定诱导型一氧化氮合酶(i NOS)活力。结果:大鼠脑缺血2h、再灌注24h后,脑组织NO水平显著升高,总NOS和i NOS活性显著增加,莲心碱可显著降低缺血再灌注损伤大鼠脑组织NO含量和总NOS、i NOS活力。结论:莲心碱可能通过降低NO对脑缺血再灌注损伤后的神经毒性作用而发挥脑缺血的保护作用。更多还原

【Abstract】 Objective:To investigate the effect of Liensinine on levels of NO and NOS in rats with cerebral ischemia-reperfusion injury.Methods:The right middle cerebral artery of the rat was occluded by inserting a thread through internal carotid artery for 2h, and then reperfused for 24h.24 male Sprague-Dawley rats were randomly divided into three groups.sham operation group, ischemia-reperfusion group, Liensinine group.Liensinine (3mg·kg-1) was injected at 30min before ischemia、after reperfusion and 12h,24h after reperfusion, tissue from the forebrain was homogenized, NO and NOS (including total NOS and iNOS) were determined. Results:The levels of NO and NOS levels were significantly increased in the brain tissue of ischemia-reperfusion group compared with shame-operate group.Compared with ischemia-reperfusion group, NO and NOS were significantly decreased in the Liensinine group .Conclusion:Liensinine can reduce ischemia-reperfusion injury by decreasing the damages of NO .更多还原