【摘要】 Mg2+是植物细胞中含量最丰富的二价阳离子,在植物体内起重要作用。在模式植物拟南芥中,已发现一个拥有10个成员的基因家族-AtMGT,其中一些成员已被鉴定出具Mg2+转运功能。实验以该家族中的At-MGT4为研究对象,将AtMGT4的cDNA与含有35S启动子的pBI121载体融合,构建过表达载体pBI121-At-MGT4。通过农杆菌浸染的方法,获得过表达植株。观察发现,部分过表达植株具有叶卷曲、花簇生、矮化和晚花等特异表型。更多还原

【Abstract】 Magnesium(Mg2+)is the most abundant divalent cation in plant cells and plays a critical role in many physiological processes in plants.Le-gong Li identitied a 10-member Arabidopsis gene family(AtMGT)encoding putative Mg2+ transport proteins.Some members of the AtMGTs were proved to have Mg2+ transport activities.This work mainly studies AtMGT4,one of the ten members of AtMGT gene family,by fusing the cDNA of AtMGT4 and pBI121 vector that contains 35 S promoter to form overexpression vector pBI121-AtMGT4.pBI121-AtMGT4 transgenetic plants were obtained by floral dip mediated by Agrobacterium.By observation,we discovered that some of the transgenetic plants had particular phenotypes,such as curling leaves,clustering clustered flowers,dwarfing and late flowering,etc.更多还原