【摘要】 应用聚丙烯酰胺凝胶电泳、同工酶分析技术分别研究了猕猴桃植株体内过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酯酶(EST)同工酶谱带的变化,结果表明:自然感染溃疡病前后,此6种同工酶谱带特征在不同抗感品种中表现出一定的差异。未感染溃疡病菌前,抗(感)品系枝条、叶片POD同工酶均有2条酶带,PPO同工酶有3条酶带,但感病品种酶带颜色深且粗,而抗病品种酶带颜色浅且细,叶片酶带颜色深于枝条;SOD、CAT同工酶谱带均为1条,Rf值分别为0.38、0.28,感性品种较抗耐品种谱带亮度高活性强;自然发病后,抗(感)品系POD、PPO同工酶谱带数都增加,分别为4、3条和5、4条,且抗病品种新酶带出现较感病品种早且酶带粗颜色深活性强,感病品系虽也有新酶带出现,但酶带少活性弱,抗病品系枝条、叶片POD、PPO同工酶新谱带的Rf值分别为0.63、0.67和0.85、0.87;抗感病品种SOD、CAT同工酶都被诱导产生了1条新的同工酶谱带,Rf分别为0.32和0.27,新酶带现色时间迟,且酶带颜色浅活性弱,但抗耐品种较感性品种谱带亮且活性强;EST同工酶于自然发病前后变化不大,与抗病性关系不很明显。更多还原

【Abstract】 The application of polyacrylamide gel electrophoresis(PAGE)and isoenzyme analytic technique to study the variations of isozyme spectra of peroxidase enzyme(POD),polyphenol oxidase(PPO),superoxide dismutase(SOD),catalase(CAT),and esterase(EST)in kiwifruit plant.The results showed that pre-and post-natural infection by Pseuomonas syringae pv.actinidae,the character of such six isozymes spectra had some differentia in susceptible and resistant cultivars.Pre-infection by Pseuomonas syringae pv.actinidae in the field,isozyme spectra of POD and PPO had 2 and 3 bands respectively in the annual twig and foliage of both susceptible and resistant cultivars,but there were also differences with regard to both width and colour,isoenzyme bands of susceptible cultivars were darker in colour and thin in width,and yet isoenzyme bands of resistant cultivars were shallower in colour and thin in width,isoenzyme bands of foliage were darker in colour than those of the annual twig,isozyme spectra of SOD and CAT all had 1 band only,relative mobilities(Rf)numerical value were 0.38,0.28 respectively,isoenzyme bands of susceptible cultivars were lighter in colour and strong in activity.Post-infection by Pseuomonas syringae pv.actinidae in the field,isozyme bands of both POD and PPO increased,having 4,3 and 5,4 respectively,and isozyme bands produced newly in resistant cultivars appeared early in time,wide in width and strong in activity,although isozyme bands produced newly in susceptible cultivars,yet they appeared late in time,few in number and weak in activity,isozyme bands of POD and PPO produced newly in the annual twig and foliage of resistant cultivars,and their Rf numerical value were 0.63,0.67 and 0.85,0.87 respectively.Isozyme of SOD and CAT were induced and produced 1 band newly in both resistant and susceptible cultivars,Rf numerical value were 0.32,0.27 respectively,new isozyme bands appeared late,light in colour and weak in activity,but isozyme bands produced newly in resistant cultivars were high in brightness and strong in activity.The change of isozyme bands of EST were not greatly in pre-and post-natural infection by Pseuomonas syringae pv.actinidae,the relation with resistance disease were not obvious.The changes of isozyme bands set up basically coherent rule with enzyme activity.All of them showed that protective enzymes defence system in cell of kiwifruit plant were actively concerned with the metabolizability function of resistance disease.更多还原