【摘要】 目的建立金乌骨通胶囊中淫羊藿苷的高效液相含量测定的方法。方法Kromasil-C18色谱柱(200mm×4.6mm,5μm),以乙腈-水(30:70,用磷酸调pH值至3.5)为流动相,检测波长为270nm。结果淫羊藿苷进样量在0.25～2.5μg范围内,与吸收峰面积呈良好的线性关系(r=0.9999)。本方法的平均回收率为97.9%。结论本法测定金乌骨通胶囊中淫羊藿苷的含量,操作简单,结果准确,重复性好。更多还原

【Abstract】 Aim To establish an HPLC method for the determination of icarrin in Jinwu Gutong capsules.Methods Icarrin was separated on the Kromasil C 18 column at 270nm with acetonitrile-water (30∶ 70) (pH adjusted to 3.5 using triethabolamine)as a mobile phase.Results A linear relationship of icarrin was observed with in the range of 0.25μg to 2.5μg.The average recovery of icarrin was 97.9%.Conclusion The method is simple,accurate and reliable for the determineation of icarrin in Jinwu Gutong capsules.更多还原